Manganese Health Research Program: Phase 2, Core 19
| Research Core Project Number: |
W81XWH-05-1-0239 |
| Research Core Project: |
In Vivo Micronucleus Assay of Manganese
According to OECD 474 Guideline and Critical
Review of the Genetic Toxicology of Manganese |
| Core Principal Investigator (CPI): |
Leslie Recio, PhD DABT
ILS, Inc
PO Box 13501
RTP, NC 27709
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| Key Collaborators: |
None
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Project Objectives:
- Conduct in vivo micronucleus assay in mice compliant with regulatory guidelines to assess the genotoxic potential of manganese chloride
- Conduct literature review of genotoxic effects of manganese
Project Description:
The purpose of this project is to assess the in vivo genotoxic potential of manganese chloride in an assay that meets international guidelines for genotoxicity assessments. The micronucleus assay in mice is the only in vivo genotoxicity test that is part of the genotoxicity test battery required by regulatory agencies worldwide. The in vivo micronucleus assay detects chromosomal damage in the peripheral blood of test species. This project will assess the in vivo genotoxicity of manganese in a study that meets the experiential design of regulatory guidelines for the conduct of the in vivo micronucleus. The second objective of this project is to conduct a review of the literature on the genotoxic potential of manganese.
Project Status:
Project started: April 15, 2008
Scheduled completion date: January 31, 2010
Anticipated completion date: September 30, 2009
Publications:
Stankowski J., Leitch D., Aschner M., McLaughlin B. and Stanwood G. D. (2008) Selective vulnerability of dopaminergic systems to Manganese: Relevance to occupational exposure. Neurotoxicology And Teratology 30, 259.
Key research accomplishments:
- Female mice are more sensitive than male mice to the acute effects of MnCl2
- A top dose of 200 mg/kg will be used to conduct a micronucleus assay in female mice in compliance with OECD 474
